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1 article(s) from Martinez Rodriguez, Brenda

Activity assays of NnlA homologs suggest the natural product N-nitroglycine is degraded by diverse bacteria

Kara A. Strickland,
Brenda Martinez Rodriguez,
Ashley A. Holland,
Shelby Wagner,
Michelle Luna-Alva,
David E. Graham and
Jonathan D. Caranto

Beilstein J. Org. Chem. 2024, 20, 830–840, doi:10.3762/bjoc.20.75

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Graphical Abstract

Scheme 1: NNG degradation by reduced NnlA.

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Figure 1: Nitrite concentrations observed in cultures of E. coli transformed with NnlA homologs or variants g...

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Figure 2: Molecular mass determination of purified NnlA homologs by A) SDS-PAGE or B) analytical size exclusi...

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Figure 3: UV–vis absorption spectra of purified NnlA homologs. All spectra were measured in 100 mM tricine, 1...

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Figure 4: Representative LC–MS EICs monitoring molecular anions of NNG (m/z 119.01 ± 100 ppm) and glyoxylate (...

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Figure 5: Alpha-fold model of Vs NnlA dimer (cyan) overlayed with Pseudomonas aeruginosa (grey; PDB: 3VOL [35,36]) t...

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Figure 6: Phylogenetic tree of NnlA homologs with accession numbers. Branch lengths correspond to amino acid ...

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Scheme 2: Acid–base equilibrium of 3-nitropropionate (3-NP) vs N-nitroglycine (NNG).

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Published 17 Apr 2024

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